Spatial patterns of extracellular enzymes: Combining X-ray computed micro-tomography and 2D zymography
نویسندگان
چکیده
منابع مشابه
X-ray micro-computed tomography
Micro-computed tomography (μCT) has been successfully used to study the cardiovascular system of mouse embryos in situ. With the use of barium as a suitablecontrast agent, blood vessels have beenimaged and analysed quantitatively such asblood volume and vessel sizes on embryosof ages 14.5 to 16.5 days old. The advantageof using this imaging modality is that it hasprovide...
متن کاملQuantification of eggshell microstructure using X-ray micro computed tomography
1. X-ray microcomputed tomography can be used to produce rapid, fully analysable, three-dimensional images of biological and other materials without the need for complex or tedious sample preparation and sectioning. We describe the use of this technique to visualise and analyse the microstructure of fragments of shell taken from three regions of chicken eggs (sharp pole, blunt pole and equatori...
متن کاملX-ray computed tomography.
X-ray computed tomography (CT), introduced into clinical practice in 1972, was the first of the modern slice-imaging modalities. To reconstruct images mathematically from measured data and to display and to archive them in digital form was a novelty then and is commonplace today. CT has shown a steady upward trend with respect to technology, performance and clinical use independent of predictio...
متن کاملAn optimization procedure for spatial and density resolution in hard X-ray micro-computed tomography
The quality of the X-ray tomogram not only depends on the spatial resolution but also on the density resolution or contrast. Based on the theory of [Nucl. Instr. and Meth. 206 (1983) 541] it is concluded that the density resolution can be substantially improved by merging of pixels, referred to as binning, prior to reconstruction. We demonstrate that the quality of a given 3D-data set, i.e. the...
متن کاملAssessment of geometrical characteristics of dental endodontic micro-instruments utilizing X-ray micro computed tomography
OBJECTIVE The aim of this study was to quantify the surface area, volume and specific surface area of endodontic files employing quantitative X-ray micro computed tomography (mXCT). MATERIAL AND METHODS Three sets (six files each) of the Flex-Master Ni-Ti system (Nº 20, 25 and 30, taper .04) were utilized in this study. The files were scanned by mXCT. The surface area and volume of all files ...
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ژورنال
عنوان ژورنال: Soil Biology and Biochemistry
سال: 2019
ISSN: 0038-0717
DOI: 10.1016/j.soilbio.2019.06.002